Tg mpeg1:mcherry
Web10 Feb 2024 · In brief, 4 dpf Tg(mpeg1:mCherry) larvae were subject to needle-stab injury and the injured region was dissected at 1, 2 and 3 dpi and tissue dissociated into a single … WebTo confirm this point, the transgenic ZF line Tg (mpeg1:mCherry) was previously exploited to visualize the interactions between macrophages and other immune cells or pathogens in vivo and to reexamine macrophage roles in inflammation, wound healing, and development, as well as their interactions with other cell types (e.g., vasculature, muscle) in vivo ; more …
Tg mpeg1:mcherry
Did you know?
WebFirst, by performing L-plastin (Lcp1) immunohistochemistry and using transgenic Tg(mpeg1.1:GFP) or Tg(mpeg1.1:mCherry) fish, we analyzed the distribution of … Web20 Jun 2024 · We first assessed macrophage heterogeneity and recruitment dynamics following larval cardiac injury. We crossed the zebrafish pan-macrophage reporter line Tg(mpeg1:GFP) with Tg(csf1ra:gal4;UAS:mCherry-NfsB) (shortened here to csf1ra: mCherry) (Figure S1 A, related to Figure 1).Csf1ra (colony stimulated factor 1 receptor) is …
Web24 Feb 2014 · In vivo cording of the R variant prevents phagocytosis. (A) Tg(mpeg1:mCherry) embryos harboring red-fluorescent macrophages were infected with GFP-expressing R variants. (Left) Maximum intensity projection of confocal images of macrophages capable of phagocytosing the M. abscessus R variant in the vascular system. Web8 Jul 2015 · We generated a transgenic line whose macrophages expressing tumour necrosis factor alpha ( tnfa ), a key feature of classically activated (M1) macrophages, …
WebDownload scientific diagram Zebrafish notochords contain intracellular vacuoles that inflate rapidly during development. (A) Cartoon of a zebrafish notochord depicting outer and inner cell ...
Web8 Sep 2024 · The proximal 1.86-kb DNA fragment of mpeg1 5′-UTR was subcloned into the 5′ region of either EGFP or mCherry, and Tol2-mediated transgenesis was performed to …
WebHan et al., 2024 - Zebrafish mafbb Mutants Display Osteoclast Over-Activation and Bone Deformity Resembling Osteolysis in MCTO Patients. Biomolecules 11(3) Full text @ Biomolecules grease for pool filterWeb6 Aug 2013 · We performed a similar analysis to track infected macrophage-lineage cells following Sal-GFP injection into Irg1-depleted and control MO-injected Tg(mpeg1:mCherry) larvae. This revealed that Irg1-depleted macrophage-lineage cells were defective in killing phagocytozed Sal-GFP, when compared to controls (Figures 5H and 5I). grease for sale in philippinesWeb(D–G) Confocal micrographs of transverse sections from 3 dpi MTZ+ Tg(mpeg1:mCherry; rpe65a:nfsB-eGFP) larval eyes. Cyan arrowheads point to MΦs/µglia (magenta) overlapping with RPE debris (green) in central–dorsal (D and E) and central–ventral (F and G) regions. Dorsal is up; anterior is left. (Scale bars, 20 μm.) grease for rack and pinionWeb12 May 2024 · To assess the behaviour of each macrophage subgroup, we imaged Tg (Mpeg1:mCherry) embryos over the course of one hour (acquisition every minute; Video 1 ). A colour-coded process was used to... chonmage armor setWebFigure Legend Snippet: ( A ) Maximum projection of a microglia (green) making contact with myelin sheaths (magenta) in a 4 dpf Tg(mpeg1:EGFP; sox10:mRFP) zebrafish spinal cord. ( B-F ) Microglia scanning behavior along myelin sheaths in the spinal cord was analyzed by confocal time-lapse imaging of 3-4 dpf Tg(mpeg1:EGFP; mbp:mCherry-CAAX) or … grease for rubber and plasticWebTg (mpeg1:mCherryCAAX) ( 1) Type engineered_plasmid Regulatory Regions mpeg1.1 ( 1) Coding Sequences CAAX ( 1) mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) … grease for polyurethane bushingsWeb4 Aug 2024 · Next, we analyzed double transgenic cd45:CFP‐NTR;runx1:mCherry embryos at 48 and 72 hpf by flow cytometry, and triple transgenic cd45:CFP‐NTR;runx1:mCherry;mpeg1:GFP embryos at 60 hpf by live imaging (Appendix Fig S3A–C). Our data clearly show that CFP and mCherry never overlap, meaning that the 7.6 … grease for scag mower