Spinach riboswitch
WebHere we describe methods to prepare and analyze riboswitch-Spinach tRNA fusions for ligand-dependent activation of fluorescence in vivo. Example procedures describe the use of the Vc2-Spinach tRNA biosensor to monitor perturbations in cellular levels of cyclic di-GMP using either fluorescence microscopy or flow cytometry. WebHere we describe methods to prepare and analyze riboswitch–Spinach tRNA fusions for ligand-dependent activation of fluorescence in vivo. Example procedures describe the use …
Spinach riboswitch
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WebJun 5, 2024 · For example, Spinach variants can be carefully fused to riboswitch aptamer domains through a stem loop called a “transducer module ,” such that fluorescence of the Spinach/DFHBI complex is achieved only in response to binding of the riboswitch ligand , as allosterically signaled through the transducer module [31,32,33]. When optimized ... WebHere we describe Spinach riboswitches, a novel class of genetically encoded me- tabolite sensor based on riboswitches, a group of naturally oc- curring ligand-binding RNAs. Spinach riboswitches use Spinach, an RNA aptamer that binds and activates the fluorescence of an otherwise nonfluorescent small-molecule fluorophore.
WebDec 12, 2024 · The adenine riboswitch was sensitive to Mg2+ concentrations and could be used as a biosensor to measure cellular Mg2+ concentrations. Additionally, the TQ3/Cy3-labeled adenine riboswitch yielded a Mg2+ concentration that was similar to that measured using a commercial assay kit. ... In recent years, spinach, mango, and broccoli aptamers … WebFeb 22, 2024 · Riboswitches are important RNA-based elements involved in rapid and precise gene regulation. Here, we present the identification of a natural thiamine-responsive riboswitch of Lactococcus lactis, a bacterium used worldwide in …
WebTo gain a better understanding of the riboswitch regulatory mechanism, to allow for more efficient riboswitch optimization and use for biosensing applications, we studied the performance of a theophylline-responsive synthetic riboswitch coupled with the superfolder green fluorescent protein (sfGFP) reporter gene in E. coli cellular extract and ... WebHere we describe methods to prepare and analyze riboswitch–Spinach tRNA fusions for ligand-dependent activation of fluorescence in vivo. Example procedures describe the use of the Vc2-Spinach tRNA biosensor to monitor perturbations in cellular levels of cyclic di-GMP using either fluorescence microscopy or flow cytometry.
WebFeb 1, 2010 · Riboswitches are regions of mRNA that directly bind metabolites, leading to alteration of gene expression. We have developed fragment-based methods to screen for compounds that bind the Escherichia...
WebMay 11, 2015 · Here we describe Spinach riboswitches, a novel class of genetically encoded metabolite sensor based on riboswitches, a group of naturally occurring ligand-binding RNAs. Spinach riboswitches use … css noticiasWebA riboswitch in Clostridium acetobutylicum regulates an adjacent gene that is not part of the same mRNA transcript. In this regulation, the riboswitch interferes with transcription of … css not importantWebSpinach riboswitches constitute a novel class of RNA-based fluo- rescent metabolite sensors that take advantage of the evolution- arily optimized affinity and selectivity seen … css no text overflowWebJan 21, 2015 · Spinach riboswitches switch between two folded conformations in which the transducer sequence is associated with either the ligand-binding pocket or Spinach. ... ... In principle, the aptamer... earls ez-beader tubingWebSep 10, 2024 · As gene expression regulatory elements, riboswitches are structurally divided into two domains—an aptamer domain sufficient for ligand binding and an expression platform regulating gene expression... earls expressWebNational Center for Biotechnology Information earls etobicokeWebFeb 8, 2024 · Riboswitches are regions in mRNAs that contain a specific evolutionarily conserved target-binding aptamer domain and an expression platform that enables the regulation of the downstream transcription or translation. css not last-child