Scrape loading dye transfer assay
WebGap junction communication, assessed with a scrape-loading dye transfer assay, was significantly increased in direct transmembrane co-cultures … WebFigure 7 Utility of the scrape loading/dye transfer assay to detect a toxin/toxicant s ability to modulate gap junctional intercellular communication. The last two images (6ngmr TPA) illustrate both the epifluorescent and phase images, respectively, of the same cells to show that the complete inhibition of GJIC was not associated with the death ...
Scrape loading dye transfer assay
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WebA scrape loading/dye transfer assay was used to assess the effects of the chemicals on GJIC. In this assay, a scratch is made in a confluent cell layer in the presence of a Lucifer Yellow (LY) solution. This fluorescent dye is taken up by damaged cells along the scrape and is passed through to the neighboring cells when they display functional ... WebFeb 7, 2024 · e Images obtained from the scrape loading (SL)/dye transfer (DT) assay after application of lucifer yellow CH dilithium salt (LY, MW 457), which transfers via the functional gap junction channels ...
WebIt employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the monitoring of its … WebSep 18, 2014 · Scrape-loading/dye transfer assay for gap junctional intercellular communication Solutions needed: HBSS + 1% BSA (50 ml; 0.5 g BSA/HBSS) PBS + 1% …
WebA scrape loading/dye transfer assay was used to assess the effects of the chemicals on GJIC. In this assay, a scratch is made in a confluent cell layer in the presence of a Lucifer … WebMay 21, 2016 · The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically … For over 35 years, biological scientists have come to rely on the research protocols … Scrape Loading/Dye Transfer Assay. Pavel Babica, Iva Sovadinová, Brad L. Upham; …
WebThe GJIC was investigated by a scrape-loading dye transfer assay. Expression of Cx43 and K3 keratin as well as BrdU-retaining nuclei were also ana-lyzed after xenotransplantation in nude mice. Results: Limbal epithelium showed mean±SD 12.4% ±14.5%positiveunitsof Cx43expressionandalowBrdU
WebJan 20, 2024 · Fluoranthene as a non-cytotoxic GJIC inhibitor assessed using the multiparametric scrape-loading-dye transfer (SL-DT) assay in rat liver progenitor WB-F344 cells (a 24-well plate format, 1-h exposure). (a) The effects were compared with the non-treated control and expressed as the fraction of control (FOC; FOC non-treated control = … hotel service salvator spol. s r.oWebFeb 1, 1987 · It employs scrape-loading to introduce a low molecular weight (MW) fluorescent dye, Lucifer yellow CH (MW 457.2) into cells in culture and allows the … like a river around a rockWebThe scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large … like a rind crosswordWebJul 31, 2024 · A scrape loading/dye transfer assay was performed to assess the GJIC function. The expression of connexins was examined by real-time reverse transcription polymerase chain reaction (RT-PCR), western blot and immunofluorescent staining. like a rich jewel in an ethiop\u0027s earWebin scrape-loading dye transfer assay [ 13] Packaging 25, 100 mg in glass bottle Bottomless glass bottle. Contents are inside inserted fused cone. Features and Benefits This … like a riddle lyrics acousticWebJul 1, 2008 · The scrape loading and dye transfer (SL/DT) method is a functional assay widely used to evaluate the level of the intercellular communication via gap junction. Initially, the evaluation of the degree of communication was done by counting the rows of fluorescent cells from the scrape. like a ring of solid gold worship songWebOne of these assays, the scrape-loading dye transfer (SLIDT) technique, is based on monitoring the transfer of the fluorescent dye Lucifer yellow from one cell into adjacent cells via functional gap junctions. The objective of our study was to evaluate and compare various approaches for quantifying results obtained with the SL/DT technique. like a river carly simon